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Amaxa nucleofection
Nucleofection, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofection+system/nucleofection/bio_rxiv__64898__2026__05__06__723175-68-4-5
Average 86 stars, based on 1 article reviews
nucleofection - by Bioz Stars, 2026-09
86/100 stars

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Transduction:

Article Title: CRISPR/Cas9 complex for introducing a functional polypeptide into cells of blood cell lineage
Article Snippet: .. See, for example, Maasho et al. “Efficient gene transfer into the human natural killer cell line, NKL, using the amaxa nucleofection system,” Journal of Immunological Methods 284 (1-2): 133-140 (2004); and Aluigi et al. “Nucleofection is an efficient non-viral transduction technique for human bone marrow derived mesenchymal stem cells,” Stem Cells 24 (2): 454-461 (2006)), both of which are incorporated herein in their entireties by this reference. ..

Article Title: Bacterial colicin-immunity protein protein purification system
Article Snippet: .. See, for example, Maasho et al. “Efficient gene transfer into the human natural killer cell line, NKL, using the amaxa nucleofection system,” Journal of Immunological Methods 284(1-2): 133-140 (2004); and Aluigi et al. “Nucleofection is an efficient non-viral transduction technique for human bone marrow derived mesenchymal stem cells,” Stem Cells 24(2): 454-461 (2006)), both of which are incorporated herein in their entireties by this reference. ..

Derivative Assay:

Article Title: CRISPR/Cas9 complex for introducing a functional polypeptide into cells of blood cell lineage
Article Snippet: .. See, for example, Maasho et al. “Efficient gene transfer into the human natural killer cell line, NKL, using the amaxa nucleofection system,” Journal of Immunological Methods 284 (1-2): 133-140 (2004); and Aluigi et al. “Nucleofection is an efficient non-viral transduction technique for human bone marrow derived mesenchymal stem cells,” Stem Cells 24 (2): 454-461 (2006)), both of which are incorporated herein in their entireties by this reference. ..

Article Title: Bacterial colicin-immunity protein protein purification system
Article Snippet: .. See, for example, Maasho et al. “Efficient gene transfer into the human natural killer cell line, NKL, using the amaxa nucleofection system,” Journal of Immunological Methods 284(1-2): 133-140 (2004); and Aluigi et al. “Nucleofection is an efficient non-viral transduction technique for human bone marrow derived mesenchymal stem cells,” Stem Cells 24(2): 454-461 (2006)), both of which are incorporated herein in their entireties by this reference. ..

Transfection:

Article Title: Increased PRL-1 in BM-derived MSCs triggers anaerobic metabolism via mitochondria in a cholestatic rat model.
Article Snippet: 1Department of Biomedical Science, CHA University, 689, Sampyeong-dong, Bundang-gu, Seongnam-si 13488, Republic of Korea; 2Research Institute of Placental Science, CHA University, Seongnam 13488, Republic of Korea; 3Department of Internal Medicine, Catholic University Medical College, Seoul 03312, Republic of Korea; 4Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam 13496, Republic of Korea

Article Title: Paxillin Mediates Sensing of Physical Cues and Regulates Directional Cell Motility by Controlling Lamellipodia Positioning
Article Snippet: GFP-paxillin (chicken paxillin in Clonetics pEGFP-C vector) was transiently transfected into 3T3 cells using Effectene (Qiagen) according to the manufacturer's protocol. .. MEFs were transfected using the Amaxa nucleofection system using MEF Kit 2 buffer (Amaxa) on setting T-20. .. GFP-paxillin (Clontech EGFP-C, chicken paxillin mRNA) constructs were grown in XL1-Blue E. coli (Stratagene) under kanamycin selection for all cloning steps.

Article Title: Increased PRL-1 in BM-derived MSCs triggers anaerobic metabolism via mitochondria in a cholestatic rat model
Article Snippet: .. In particular, nonviral electroporation, a known AMAXA nucleofection system, demonstrated high transfection efficiency in a population of viable MSCs using a plasmid containing the cytomegalovirus (CMV) promoter. ..

Control:

Article Title: Increased PRL-1 in BM-derived MSCs triggers anaerobic metabolism via mitochondria in a cholestatic rat model.
Article Snippet: 1Department of Biomedical Science, CHA University, 689, Sampyeong-dong, Bundang-gu, Seongnam-si 13488, Republic of Korea; 2Research Institute of Placental Science, CHA University, Seongnam 13488, Republic of Korea; 3Department of Internal Medicine, Catholic University Medical College, Seoul 03312, Republic of Korea; 4Department of Gastroenterology, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam 13496, Republic of Korea

Electroporation:

Article Title: Pushing the frontiers of babesiosis research: in vitro culture and gene editing.
Article Snippet: This article provides a detailed account of gene-editing technologies, including CRISPR/Cas9 and conditional knockout/knockdown systems, and discusses the challenges and solutions associated with achieving successful gene replacement events in Babesia species through Babesiosis is a tick-borne parasitic disease that poses a significant risk to both animal and human health.. A comprehensive understanding of Babesia biology necessitates the application of advanced laboratory techniques.. This review explores recent advancements in gene editing technologies of Babesia, emphasizing the foundational importance of in vitro culture systems.

Article Title: Increased PRL-1 in BM-derived MSCs triggers anaerobic metabolism via mitochondria in a cholestatic rat model
Article Snippet: .. In particular, nonviral electroporation, a known AMAXA nucleofection system, demonstrated high transfection efficiency in a population of viable MSCs using a plasmid containing the cytomegalovirus (CMV) promoter. ..

Plasmid Preparation:

Article Title: Increased PRL-1 in BM-derived MSCs triggers anaerobic metabolism via mitochondria in a cholestatic rat model
Article Snippet: .. In particular, nonviral electroporation, a known AMAXA nucleofection system, demonstrated high transfection efficiency in a population of viable MSCs using a plasmid containing the cytomegalovirus (CMV) promoter. ..

CRISPR:

Article Title: Elevated FOXG1 in glioblastoma stem cells cooperates with Wnt/β-catenin to induce exit from quiescence.
Article Snippet: .. Design and construction of CRISPR sgRNAs is described in.60 The Amaxa (Lonza) nucleofection system was used. ..



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